H2N2V1, Main, Exploration, bibRecord, 000A72

Comprehensive global amino acid sequence analysis of PB1F2 protein of influenza A H5N1 viruses and the influenza A virus subtypes responsible for the 20th‐century pandemics

Identifieur interne : 000A72 ( Main/Exploration ); précédent : 000A71; suivant : 000A73

Comprehensive global amino acid sequence analysis of PB1F2 protein of influenza A H5N1 viruses and the influenza A virus subtypes responsible for the 20th‐century pandemics

Auteurs : Gunisha Pasricha ; Akhilesh C. Mishra ; Alok K. Chakrabarti

Source :

Influenza and Other Respiratory Viruses [ 1750-2640 ] ; 2012.

RBID : PMC:5780999

Descripteurs français

KwdFr :
- Alignement de séquences, Analyse de regroupements, Analyse de séquence de protéine, Animaux, Biologie informatique (), Grippe humaine (virologie), Grippe humaine (épidémiologie), Humains, Infections à Orthomyxoviridae (médecine vétérinaire), Infections à Orthomyxoviridae (virologie), Infections à Orthomyxoviridae (épidémiologie), Pandémies, Phylogénie, Protéines mutantes (génétique), Protéines virales (génétique), Substitution d'acide aminé, Variation des antigènes, Variation génétique, Virus de la grippe A (génétique), Virus de la grippe A (isolement et purification).
MESH :
- génétique : Protéines mutantes, Protéines virales, Virus de la grippe A.
- isolement et purification : Virus de la grippe A.
- médecine vétérinaire : Infections à Orthomyxoviridae.
- virologie : Grippe humaine, Infections à Orthomyxoviridae.
- épidémiologie : Grippe humaine, Infections à Orthomyxoviridae.
- Alignement de séquences, Analyse de regroupements, Analyse de séquence de protéine, Animaux, Biologie informatique, Humains, Pandémies, Phylogénie, Substitution d'acide aminé, Variation des antigènes, Variation génétique.

English descriptors

KwdEn :
- Amino Acid Substitution, Animals, Antigenic Variation, Cluster Analysis, Computational Biology (methods), Genetic Variation, Humans, Influenza A virus (genetics), Influenza A virus (isolation & purification), Influenza, Human (epidemiology), Influenza, Human (virology), Mutant Proteins (genetics), Orthomyxoviridae Infections (epidemiology), Orthomyxoviridae Infections (veterinary), Orthomyxoviridae Infections (virology), Pandemics, Phylogeny, Sequence Alignment, Sequence Analysis, Protein, Viral Proteins (genetics).
MESH :
- chemical , genetics : Mutant Proteins, Viral Proteins.
- epidemiology : Influenza, Human, Orthomyxoviridae Infections.
- genetics : Influenza A virus.
- isolation & purification : Influenza A virus.
- methods : Computational Biology.
- veterinary : Orthomyxoviridae Infections.
- virology : Influenza, Human, Orthomyxoviridae Infections.
- Amino Acid Substitution, Animals, Antigenic Variation, Cluster Analysis, Genetic Variation, Humans, Pandemics, Phylogeny, Sequence Alignment, Sequence Analysis, Protein.

Abstract

Please cite this paper as: Pasricha et al. (2012) Comprehensive global amino acid sequence analysis of PB1F2 protein of influenza A H5N1 viruses and the Influenza A virus subtypes responsible for the 20th‐century pandemics. Influenza and Other Respiratory Viruses 7(4), 497–505.

Background PB1F2 is the 11th protein of influenza A virus translated from +1 alternate reading frame of PB1 gene. Since the discovery, varying sizes and functions of the PB1F2 protein of influenza A viruses have been reported. Selection of PB1 gene segment in the pandemics, variable size and pleiotropic effect of PB1F2 intrigued us to analyze amino acid sequences of this protein in various influenza A viruses.

Methods Amino acid sequences for PB1F2 protein of influenza A H5N1, H1N1, H2N2, and H3N2 subtypes were obtained from Influenza Research Database. Multiple sequence alignments of the PB1F2 protein sequences of the aforementioned subtypes were used to determine the size, variable and conserved domains and to perform mutational analysis.

Results Analysis showed that 96·4% of the H5N1 influenza viruses harbored full‐length PB1F2 protein. Except for the 2009 pandemic H1N1 virus, all the subtypes of the 20th‐century pandemic influenza viruses contained full‐length PB1F2 protein. Through the years, PB1F2 protein of the H1N1 and H3N2 viruses has undergone much variation. PB1F2 protein sequences of H5N1 viruses showed both human‐ and avian host‐specific conserved domains. Global database of PB1F2 protein revealed that N66S mutation was present only in 3·8% of the H5N1 strains. We found a novel mutation, N84S in the PB1F2 protein of 9·35% of the highly pathogenic avian influenza H5N1 influenza viruses.

Conclusions Varying sizes and mutations of the PB1F2 protein in different influenza A virus subtypes with pandemic potential were obtained. There was genetic divergence of the protein in various hosts which highlighted the host‐specific evolution of the virus. However, studies are required to correlate this sequence variability with the virulence and pathogenicity.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780999

DOI: 10.1111/j.1750-2659.2012.00400.x
PubMed: 22788742
PubMed Central: 5780999

Affiliations:

Le document en format XML

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<term>Animals</term>
<term>Antigenic Variation</term>
<term>Cluster Analysis</term>
<term>Computational Biology (methods)</term>
<term>Genetic Variation</term>
<term>Humans</term>
<term>Influenza A virus (genetics)</term>
<term>Influenza A virus (isolation & purification)</term>
<term>Influenza, Human (epidemiology)</term>
<term>Influenza, Human (virology)</term>
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<term>Biologie informatique ()</term>
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<term>Grippe humaine (épidémiologie)</term>
<term>Humains</term>
<term>Infections à Orthomyxoviridae (médecine vétérinaire)</term>
<term>Infections à Orthomyxoviridae (virologie)</term>
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<term>Virus de la grippe A (isolement et purification)</term>
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<term>Viral Proteins</term>
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<term>Orthomyxoviridae Infections</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A virus</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines mutantes</term>
<term>Protéines virales</term>
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<keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Infections à Orthomyxoviridae</term>
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<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Grippe humaine</term>
<term>Infections à Orthomyxoviridae</term>
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<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Influenza, Human</term>
<term>Orthomyxoviridae Infections</term>
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<term>Infections à Orthomyxoviridae</term>
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<term>Antigenic Variation</term>
<term>Cluster Analysis</term>
<term>Genetic Variation</term>
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<term>Pandemics</term>
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<term>Sequence Alignment</term>
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<term>Analyse de regroupements</term>
<term>Analyse de séquence de protéine</term>
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<term>Biologie informatique</term>
<term>Humains</term>
<term>Pandémies</term>
<term>Phylogénie</term>
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<front><div type="abstract" xml:lang="en"><p><italic>Please cite this paper as:</italic>
 Pasricha <italic>et al.</italic>
 (2012) Comprehensive global amino acid sequence analysis of PB1F2 protein of influenza A H5N1 viruses and the Influenza A virus subtypes responsible for the 20th‐century pandemics. Influenza and Other Respiratory Viruses 7(4), 497–505.</p>
<p><bold>Background </bold>
 PB1F2 is the 11th protein of influenza A virus translated from +1 alternate reading frame of PB1 gene. Since the discovery, varying sizes and functions of the PB1F2 protein of influenza A viruses have been reported. Selection of PB1 gene segment in the pandemics, variable size and pleiotropic effect of PB1F2 intrigued us to analyze amino acid sequences of this protein in various influenza A viruses.</p>
<p><bold>Methods </bold>
 Amino acid sequences for PB1F2 protein of influenza A H5N1, H1N1, H2N2, and H3N2 subtypes were obtained from Influenza Research Database. Multiple sequence alignments of the PB1F2 protein sequences of the aforementioned subtypes were used to determine the size, variable and conserved domains and to perform mutational analysis.</p>
<p><bold>Results </bold>
 Analysis showed that 96·4% of the H5N1 influenza viruses harbored full‐length PB1F2 protein. Except for the 2009 pandemic H1N1 virus, all the subtypes of the 20th‐century pandemic influenza viruses contained full‐length PB1F2 protein. Through the years, PB1F2 protein of the H1N1 and H3N2 viruses has undergone much variation. PB1F2 protein sequences of H5N1 viruses showed both human‐ and avian host‐specific conserved domains. Global database of PB1F2 protein revealed that N66S mutation was present only in 3·8% of the H5N1 strains. We found a novel mutation, N84S in the PB1F2 protein of 9·35% of the highly pathogenic avian influenza H5N1 influenza viruses.</p>
<p><bold>Conclusions </bold>
 Varying sizes and mutations of the PB1F2 protein in different influenza A virus subtypes with pandemic potential were obtained. There was genetic divergence of the protein in various hosts which highlighted the host‐specific evolution of the virus. However, studies are required to correlate this sequence variability with the virulence and pathogenicity.</p>
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Comprehensive global amino acid sequence analysis of PB1F2 protein of influenza A H5N1 viruses and the influenza A virus subtypes responsible for the 20th‐century pandemics

Comprehensive global amino acid sequence analysis of PB1F2 protein of influenza A H5N1 viruses and the influenza A virus subtypes responsible for the 20th‐century pandemics

Source :

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Abstract

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